Description

NGS DNA Library Prep Kit (illumina and MGI Platforms)

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most of DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.

Some genomic regions are very difficult to be covered evenly and usually result in very low coverage rate or gap in these regions.

Typical difficult regions are:• with high GC contents• have secondary structures: mainly due to repeat sequences• the worst cases: have both high GC contents and repeated sequences.

Example: human TERT gene is one of the most difficult regions as shown above. NGS data showed that BioDynami kit has the best performance to cover the extremely difficult human TERT gene region.

Limited GC bias across whole genome

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30009): Libraries do not have index.

Index (illumina Cat.# 30021): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples.

Unique dual index (illumina Cat.# 30023): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors.

Indexes are available for the MGI platform kits (Cat.# 34021).

Related Products

• PCR-free NGS DNA Library Prep Kit
• NGS Low Input DNA Library Prep Kit
• ChIP-Seq Library Prep Kit
• NGS Cell-free DNA Library Prep Kit
• NGS FFPE DNA Library Prep Kit
• Bisulfite Sequencing Library Prep Kit
• Methylation Specific Bisulfite-Seq Library Prep Kit
• NGS Single Stranded DNA Library Prep Kit
• NGS Ancient DNA Library Prep Kit
• NGS DNA Fragmentation & Library Prep Kit
• RNA Seq Library Prep Kit
• NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)
• Multiplexing Index Primers (Illumina Platform)
• Multiplexing Unique Dual Index Primers (Illumina Platform)

BioDynami是一家生物技术公司，为生命科学研究提供创新的解决方案。我们专注于下一代测序（NGS）库制备领域。我们已经开发了几种独特的技术，这些技术构成了BioDynami的基础。我们为生物医学科学家提供高质量，快速，简单和负担得起的NGS试剂。BioDynami的NGS库制备试剂涵盖主要的测序平台，不同的应用程序和各种输入资源。

我们的团队在创新技术开发和成功的业务管理方面拥有良好的记录。我们期待将新技术和试剂添加到我们的产品组合中。我们相信，高质量，快速，简单和负担得起的试剂将使科学家更容易进行研发。